Create accountLogin

Recent searches

No recent searches

Search options

Only available when logged in.
mamot.fr is one of the many independent Mastodon servers you can use to participate in the fediverse.
Mamot.fr est un serveur Mastodon francophone, géré par La Quadrature du Net.

Administered by:

Server stats:

3.1K
active users

mamot.fr: AboutStatusProfiles directoryPrivacy policy

Mastodon: AboutGet the appKeyboard shortcutsView source codev4.3.4

#microalgae

0 posts0 participants0 posts today
Public
Ajay Iyer

Happy to share my latest preprint: "Using network component analysis to study axenisation strategies for phototrophic eukaryotic microalgae"

(DOI: dx.doi.org/10.1101/2025.03.07.).

We revisited all publications in the last century focusing on the axenisation of microalgae, and used Network Component Analysis to understand how workflows can be (re-)desgined to remove contaminating microbes from microalgal cultures.

#preprint #microalgae @academicchatter @academia

Public
Lukas VFN 🇪🇺

How Does Life Happen When There’s Barely Any Light? quantamagazine.org/how-does-li

Photosynthetic light requirement near the theoretical minimum detected in Arctic #microalgae: Clara Hoppe et al. nature.com/articles/s41467-024

"Under the sea ice during the #Arctic’s pitch-black polar night, #algae power #photosynthesis on the lowest light levels ever observed in nature."

Simple illustration of algae of various geometric shapes under ice at night. One of them is shining bright green and white.
Public
Lukas VFN 🇪🇺

#Phytochromes: The 'eyes' that enable #microalgae to find their way in aquatic depths phys.org/news/2024-12-phytochr

#Diatom phytochromes integrate the underwater light spectrum to sense depth: Carole Duchêne et al. nature.com/articles/s41586-024

"These photoreceptors enable them to detect changes in the light spectrum in the water column, thereby providing information regarding their vertical position within it."

microscopic photo of a spherical single-celled alga
#Protists#Algae#Diatoms
Public *
Ajay Iyer

Contaminated microalgal cultures; especially with multidrug-resistant bacteria, pose a challenge for researchers.
Our preprint," Axenisation of Oleaginous Microalgal Cultures via Anoxic Photosensitisation", described a new method using anaerobic conditions and Rose Bengal to decontaminate cultures without antibiotics.
DOI: doi.org/10.1101/2024.11.07.622

Do share with other algae researchers here, as I would love to connect with them.

#academia #microalgae #openscience @academia @academicchatter

bioRxiv · Axenisation of oleaginous microalgal cultures via anoxic photosensitisationGrowing interest in sustainable biofuel research has necessitated high quality axenic oleaginous microalgal strains. Unfortunately, most strains available in culture banks contain commensal microbes such as bacteria and the default decontamination method involves antibiotic treatment which has begun to exacerbate the emergence of antibiotic resistance. To overcome this problem, anoxic photosensitisation was investigated as an alternate approach. Four oleaginous microalgal species ( Tetradesmus obliquus, Desmodesmus armatus, Chlorella vulgaris and Nannochloropsis limnetica ) were incubated in varying concentrations of Rose Bengal (0 µM, 1 µM, 3 µM and 9 µM) either in normal (oxic) or anoxic conditions, for 72 h under light (8.85 ± 0.40 W m−2) in a specially designed heterotrophic growth complex (HGC) medium, followed by 72 h in standard Bold’s Basal Medium (BBM). Commonly used antibiotics-based protocol was used as the control method. Post treatment, cell numbers and percentage populations were counted with Flow cytometry, and viability was tested using standard plating methods using BBM and LB. Additionally, the contaminating microbes in the cultures were profiled using 16s rRNA sequencing. Anoxic conditions were able to significantly decrease bacterial content, albeit with an equally detrimental effect on the microalgal population. Although the responses differed between the microalgae, anoxic incubation along with Rose Bengal at 3 µM was able to completely decontaminate N. limnetica and C. vulgaris , while D. armatus and T. obliquus could be decontaminated with an additional streak-plating step. None of the cultures could be decontaminated using antibiotics treatment owing to the presence of gram-negative, multi-drug resistant bacteria such as S. maltophilia, M. foliorum and S. chilensis . These results suggest that decontamination of xenic microalgal cultures was largely due to anoxy, that was synergistically enhanced by Rose Bengal at a concentration of ≥3 µM. Highlights 1. Rose Bengal at 3 µM in anoxic conditions can help achieve axenic microalgal cultures. 2. Standard antibiotics were unable to decontaminate any of the cultures owing to the presence of multi-drug resistant bacteria. 3. Standard antibiotics treatment was deleterious to D. armatus cultures. 4. Streak plating may be required for D. armatus and T. obliquus after anoxic Rose Bengal treatment to obtain individual colonies for further inoculation. ### Competing Interest Statement The authors have declared no competing interest.
Public *
Marie Walde

The #Roscoff Culture Collection 🇨🇵 of #marine #microorganisms 🌊 🦠 is seeking a #postdoc to work on ex situ microbiome interactions (EU projects #MICROBE and #BlueRemediomics 🇪🇺).

One objective is to define synthetic #microbiomes to promote the growth of selected #microeukaryotes (#microalgae, saprotrophs) and for optimal production of #metabolites of interest.

roscoff-culture-collection.org
microbeproject.eu
blueremediomics.eu
#protists #microbiology #job #bretagne #france

The selected candidate will work under the supervision of Charles Bachy (R&D Director of the RCC), Priscillia Gourvil (Technical Director of the RCC) and lan Probert (Director of the CRBM). Applications: Send a motivation letter and a detailed CV with the names of 2 references before the 1/1/2025 to rcc@sb-roscoff.fr. Interview: planned in Roscoff (or by videoconference) before the 15th January 2025.
ExploreLive feeds
About